{"id":489,"date":"2025-10-15T18:17:30","date_gmt":"2025-10-15T09:17:30","guid":{"rendered":"https:\/\/neuroclub.jikei-neuroscience.com\/?p=489"},"modified":"2025-10-15T18:18:17","modified_gmt":"2025-10-15T09:18:17","slug":"2025-10-15","status":"publish","type":"post","link":"https:\/\/neuroclub.jikei-neuroscience.com\/?p=489","title":{"rendered":"2025-10-15"},"content":{"rendered":"\n<p>\u85ac\u7406\u5b66\u8b1b\u5ea7\u306e\u9234\u6728\u3067\u3059\u3002<br>10\u670815\u65e5\u306eNC\u3067\u306f\u3001\u4e0b\u8a18\u306e\u8ad6\u6587\u3092\u7d39\u4ecb\u3044\u305f\u3057\u307e\u3059\u3002<br><a href=\"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/full\/10.1113\/JP286735\">Ageing-induced weakness of mouse NMJs is associated with reduced active<br>zone density, synaptic event kinetics and presynaptic calcium entry.<br>Yizhi Li, Elinor H. Case, Christopher Blanchard, Anna Monteleone, Meera<br>Gandhi, Anousha Jaie, Yomna Badawi, Stephen D. Meriney<br>Journal of Physiology, 2025<\/a><\/p>\n\n\n\n<p><strong>Abstract<br><\/strong>Ageing has been shown to affect both the structure and function of the<br>neuromuscular junction (NMJ). In our previous study, we documented a<br>biphasic change (first an increase followed by a decrease) in<br>neurotransmission over the ageing time course at male mouse NMJs. Here,<br>we explored several potential mechanisms behind the reduction in<br>presynaptic neurotransmitter release in the later stages of ageing. We<br>found that the active zone (AZ) density significantly decreased in NMJs<br>at 30 months, compared to 4- and 26-month-old mice. Furthermore, the<br>decreased end plate potential (EPP) amplitude in these 30-month-old mice<br>was associated with a significantly longer rise and decay time, and,<br>although miniature end plate potential (mEPPs) amplitude and frequency<br>were unchanged, these events also had a longer rise and decay time.<br>Thirty-month-old NMJs also showed a significant reduction in the vesicle<br>replenishment rate (VRR). Additionally, 30\u201333-month-old NMJs had reduced<br>presynaptic calcium entry following a single presynaptic action<br>potential. Taken together, these transmitter release site changes may<br>explain age-induced reductions in neuromuscular neurotransmission in<br>aged mice and may also lead to the identification of novel therapeutic<br>targets or potential biomarkers for future research in this area.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u85ac\u7406\u5b66\u8b1b\u5ea7\u306e\u9234\u6728\u3067\u3059\u300210\u670815\u65e5\u306eNC\u3067\u306f\u3001\u4e0b\u8a18\u306e\u8ad6\u6587\u3092\u7d39\u4ecb\u3044\u305f\u3057\u307e\u3059\u3002Ageing-induced weakness of mouse NMJs is associated with reduced activezo&#8230;<\/p>\n","protected":false},"author":1,"featured_media":491,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[3],"tags":[],"class_list":["post-489","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-journal-club"],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"https:\/\/neuroclub.jikei-neuroscience.com\/wp-content\/uploads\/2025\/10\/image-1.png","_links":{"self":[{"href":"https:\/\/neuroclub.jikei-neuroscience.com\/index.php?rest_route=\/wp\/v2\/posts\/489","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/neuroclub.jikei-neuroscience.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/neuroclub.jikei-neuroscience.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/neuroclub.jikei-neuroscience.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/neuroclub.jikei-neuroscience.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=489"}],"version-history":[{"count":1,"href":"https:\/\/neuroclub.jikei-neuroscience.com\/index.php?rest_route=\/wp\/v2\/posts\/489\/revisions"}],"predecessor-version":[{"id":490,"href":"https:\/\/neuroclub.jikei-neuroscience.com\/index.php?rest_route=\/wp\/v2\/posts\/489\/revisions\/490"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/neuroclub.jikei-neuroscience.com\/index.php?rest_route=\/wp\/v2\/media\/491"}],"wp:attachment":[{"href":"https:\/\/neuroclub.jikei-neuroscience.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=489"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/neuroclub.jikei-neuroscience.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=489"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/neuroclub.jikei-neuroscience.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=489"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}